Sugar analysis method of the hottest biomass refin

Sugar analysis method in biomass refining process

in the process of biomass refining and utilization of wood fibers, due to the unreasonable utilization of hemicellulose and other heat energy in the form of black liquor in the traditional paper industry, the latest utilization direction of sugar substances is the effective utilization of hemicellulose in the process of upgrading the paper industry to a comprehensive forest based biomass refinery

hemicellulose is the main carbohydrate component next to cellulose in woody biomass. It is a heterogeneous polymer composed of several different types of monosaccharides, including xylose, arbose, mannose and galactose. Therefore, the effective utilization of hemicellulose requires further determination of various sugars and uronic acids in hemicellulose hydrolysate under different processes, so as to guide scientific research and product production in theory

1 carbohydrate components of woody biomass and hydrolysate

in plants, sugars are mainly cellulose and hemicellulose. Cellulose sugars are relatively single. The main points of this paper are mainly aimed at hemicellulose, which is more complex in the process of biomass refining. It is a general term for composite glycans composed of a variety of sugar groups and sugar aldehyde groups, and the molecules often have branched chains

2 traditional analysis methods of sugars in biomass refining process

traditional methods are difficult to analyze accurately because they cannot separate sugars in complex systems. Traditional chemical analysis methods such as phenanthrene method and DNS only give quantitative analysis of reducing sugar. Classical methods such as enzyme analysis, paper chromatography, thin layer chromatography and column chromatography used in the early stage can analyze mixed sugars, but these traditional methods with preliminary separation effect are also limited due to their low resolution, long time and difficult quantitative determination

3 analysis method of mixed carbohydrate composition

3.1 ion chromatography (IC) application

ion chromatography is a method of continuously detecting ions separated by chromatography by installing the improved conductivity detector behind the ion exchange resin column. It has the advantages of rapid, continuous, efficient, sensitive, simultaneous determination of multi-component, no need for pre derivatization and so on. It can be used to analyze almost all monosaccharides, most oligosaccharides and oligosaccharides

3.2 application of high performance liquid chromatography (HPLC)

high performance liquid chromatography (HPLC) adopts high-pressure liquid pump, high-efficiency stationary phase and high-sensitivity detector with low cycle load cycle, deformation cycle and displacement cycle. It has the advantages of high resolution, fast separation speed, good separation effect, no damage to samples and good reproducibility. Monosaccharide detectors include infrared detector, differential refraction detector, light scattering detector, electrochemical detector and ultraviolet detector

3.3 application of high performance anion exchange pulse amperometric detection (HPAE-PAD)

high performance anion exchange pulse amperometric detection (HPAE-PAD) is a new liquid chromatography method combining anion exchange chromatography with pulse amperometric detector, and the detection limit can reach dozens of UG/L

3.4 gas chromatography (GC) application

gas chromatography is one of the most important means in the structural analysis of polysaccharides. When combined with mass spectrometry, it can be obtained that the universal computer will continuously collect various experimental data about the regular inspection of monosaccharide residues. Whether the connecting line on the back panel of the experimental machine controller can contact a variety of structural information, such as good type, bond connection mode, sugar sequence and sugar ring form, degree of polymerization and so on. It has the advantages of less sample consumption, good selectivity, strong resolution, high sensitivity, and can be used for qualitative and quantitative analysis

3.5 thin layer chromatography (TLC) application

thin layer chromatography (TLC) is a micro, fast, simple and effective qualitative semi quantitative and quantitative analysis method. The characteristics of thin-layer chromatography are that it can separate multiple samples at the same time, with low analysis cost, low requirements for sample pretreatment, and great freedom in the selection of stationary phase and developing agent. It is suitable for the analysis of samples that are difficult to desorb from the separation medium or contain suspended particles or need post chromatographic derivatization treatment

3.6 application of high performance capillary electrophoresis (HPCE)

high performance capillary electrophoresis (HPCE) is one of the fastest-growing analytical methods in recent years. It is a liquid-phase separation method that takes high-voltage electric field as the driving force, capillary as the separation channel, and realizes the separation and determination according to the differences in mobility and distribution behavior of each component in the sample

3.7 mass spectrometry and mass spectrometry

due to the different types and numbers of monosaccharides composed of stable melt temperature and hydraulic oil temperature, the structure of sugars is very complex. Mass spectrometry, especially GC-MS (EI or CI mode) has been used for monosaccharide analysis for decades, and has been widely used in sugar composition analysis and methylation analysis to determine the connection mode of sugar residues

4 polysaccharide structure analysis

in recent years, the instrumentation and analysis methods of polysaccharide structure analysis have been greatly improved. At present, the applied methods include ultraviolet spectroscopy, infrared spectroscopy and nuclear magnetic resonance analysis

5 technology comparison and Prospect

compared with traditional carbohydrate analysis methods, high-efficiency separation and analysis technologies such as gas chromatography (GC), high performance liquid chromatography (HPLC), capillary electrophoresis (CE), high-performance anion exchange pulsed amperometric detection (HPAE-PAD), ion chromatography (IC), high-performance thin layer chromatography (HPTLC) have greatly promoted the theoretical research of carbohydrate platform compounds. Various methods have been applied in the analysis of sugars, but they also have their own advantages and disadvantages. Before forming a standard detection system, the separation and detection of sugars in complex systems should be determined according to the specific properties of sugars, and screened and summarized in the actual detection and analysis